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Advanced Research Workshop
Human Monitoring for Genetic Effects

June 23-27, 2002, Kraków, Poland

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Day 1st
23rd of June 2002
10:30-13:00  Registration, accommodation, organizing discussion groups
Sightseeing: Royal Castle - Wavel
12:30-13:30  Lunch at the restaurant "Sinus"
13:30-14:00  Walk through Museum part of the Collegium Maius
14:00-15:00  Aula Collegium Maius of the Jagiellonian University - OPENING CEREMONY
15:00-16:30  Session: "Human monitoring for genetic effects"
P.Bobrzyñski Lecture Hall - Collegium Maius
Chair persons: D.Anderson, H.Autrup

W.Au "Human population monitoring for risk in environmental disease: experience with Alexander Hollaender activities
W.Jêdrychowski, Molecular epidemiology - rationale and expectations.
E. Zeiger, Ethical issues in human monitoring studies. What should the subjects be told, and what are their rights?
16:30-17:00  Coffee break
17:00-18:30  Session: "Biomarkers of exposure"
Chair persons: E.Zeiger, B.Binkova

K. Pucha³a, What can be done by industry to decrease health risk?
H. Autrup, Adverse health effects of ambient air pollution - focus on exposure assessment, including the use of biomarkers.
R.Šrám, Molecular epidemiology studies on effects of air pollution.
R.Tice, Detecting the presence of genotoxic substances in body fluids.
19:00 Dinner at "Sinus".

Day 2nd
24th of June 2002
Aula at the Henryk Niewodniczañski Institute of Nuclear Physics.
8:30-10:30  Session: "Environment and Human Health"
Chair persons: N.Holland, R.Šrám.

K.A.Karimov, Effects of unfavorable environment impact in high altitude conditions.
K.Chomiczewski, Health, genetic hazard from new biological weapons and risks.
M.Sısiadek, Clinical effects of exposure to mutagens.
D.Anderson, Review of congenital malfprmations and cancer in human and rodent offspring after paternal exposure to chemicals and radiation.
10:30-11:00  Coffee break
11:00-12:30  Session: "Biomarkers of effects"
Chair persons: M.Sasiadek, W.Au

S. Bonassi, Cytogenetic biomarkers and human health.
D. Anderson, Examination of biomarkers in humans after occupational exposure to chemicals.
N.T. Holland, Human biomonitoring using micronucleus assay in blood and exfoliated cells.
H.N. El-Khatib, Cytogenetic biomonitoring of Egyptian workers exposed to pesticides: micronuclei analysis in peripheral blood lymphocytes.
12:30-14:30  Lunch break
14:30-16:00  Session: "Biomarkers of susceptibility"
Chair persons: A.Cebulska-Wasilewska, R.Tice

D.Bell, Incorporating genetic polymorphism data into the risk assessment process.
H. Autrup, Genetic polymorphism in xenobiotica metabolising enzymes as risk factors in chronic diseases.
R.Tice, Detection of individuals with increased susceptibility by virtue of differences in DNA repair competency using the Comet assay.
W. W.Au, Susceptibility factors and chromosome aberrations for identifying the risk for environmental cancer.
16:00-16:30  Coffee break
16:30-17:30  Poster Session - stand by presentation
17:30-18:30  Round table discussion I "Poster Session"
Chair persons: D.Bell, K.Karimov
18:30 Dinner at INP

Day 3rd
25th of June 2002
Aula and lecture rooms at the Henryk Niewodniczañski Institute of Nuclear Physics.
9:00-12:30  Laboratory demonstrations and practical training for molecular and cytogenetic procedures:
  • Dose response relationship for chromosome aberrations induced by radiation
  • Analysis of variation in the response to chemicals of the biomarkers detected in second mitosis (SCE, HFC, PRI).
  • Capacity and kinetics of repair of the DNA damage induced by challenging dose of X-rays and evaluated by Comet assay.
  • Interaction of chemicals and radiation in the induction of gene mutations.
Responsible: scientist from Department of Environmental and Radiation Biology, Institute of Nuclear Physics, Kraków.

12:30-14:30  Lunch break
14:00-16:00  Laboratory demonstrations and practical training for molecular and cytogenetic procedures: (CA, MN, FISH, SCGE, optimation of the cytogenetic measures, presentations: Microptic, Kinetic, Olympus, Swetherm).
16:00-16:30  Coffee break
16:30-18:00  Round table discussion III "Role of standardization in laboratory procedures"
Chair persons: R.Tice, S.Bonassi
18:30 Dinner at INP

Day 4th
26th of June 2002
8:30-10:30  Session "Molecular biology in cancer"
Chair persons: N.Holland, S.Bonassi

W.NiedŸwiedŸ, The DNA microarray in molecular epidemiology.
I.Kalina, Molecular biology in lung cancer.
D.Anderson, Ras p21 proteins in plasma from workers exposed to chemicals by comparison with patients with lung disease.
B.Binková, P. Rössner, M. Dostál, R.J. Šrám, The effect of polycyclic aromatic hydrocarbons on human diploid lung fibroblasts: DNA adducts, expression of p53 and p21WAF1 proteins and cell cycle.
10:30-11:00  Coffee break
11:00-12:30  Session: "Molecular epidemiology study: design and analysis of results"
Chair persons: B.Binkova, H.N.El-Khatib

S.Bonassi, Cohort studies, design and outcome.
W.Jedrychowski, Bias in epidemiological studies.
A.Cebulska-Wasilewska, Extrapolation from high to low doses or exposures.
E.Zeiger, Publication issues for human monitoring studies; data compilation and presentation; the least publishable unit.
12:30-14:00  Lunch break
14:00-16:00  Session: "Molecular epiemiology study: design and analysis of results"
Chair persons: N.Holland, D.Bell

A.Cebulska-Wasilewska, Challenging dose as a predictive assay for molecular epidemiology.
Ch.Schonwalder, Joining toxicology and epidemiology for better risk assessment.
D.Anderson, Discussion on WHO/IPCS guidelines for human monitoring.
NATO, "The role and need for joint research in human monitoring studies".
16:00-16:30  Coffee break
16:30-18:00  Round table discussion II "Role and need for joint research in human monitoring studies"
Chair persons: E.Zeiger, R.Šrám.
19:00  Conference dinner

Day 5th
27th of June 2002
9:00-15:00  Excursion - Zakopane - Discussion with Dr Pawe³ Skawiñski - Director of Tatra Mountains National Park - "New ecological energy supply - expectation of the environmental health improvement"
16:00  Closing ceremony

Poster Presentations

O. Beskid, B. Binkova, P. Rossner, R.J. Šrám, Fish analysis of chromosome breakage as biomarker of genotoxicity of urban air pollution

E. Biros, O. Beskid, P. Rossner, R.J. Šrám, Genotype frequencies of the hOGG1 Ser326Cys polymorphism in the Czech male population

O. Dalivelya, Chemical clasto- and anticlastogenicity in mouse micronucleus test

L.M. Deac, Various staphylococcal infections and the circulation of phagolisotypes in several clinical units

Z. Jirák, H. Tomásková, H. Lehocká, L. Dobiás, J. Horáèek, Exposure to dusts containing quartz and human carcinogenicity

M.M. Dobrzyñska, The effects of subchronic exposure to low doses of X-rays on the reproduction ability

A.-V. Domkiene, Saule Uleckiene, Effect of vitamin E on benzo(a)pyrene induced micronuclei in vivo

V.Dulskiene, R. Grazuleviciene, Association between exposure to LEAD and risk of congenital heart malformations among smoking mothers.

Z. Dusek, B. Novotna, L. Vodickova, I. Naprstkova, M. Dostal, M. Vilhelmova, The effects of 1,2-Dibromoethane on the hematopoiesis of the chick embryo

A.Cebulska-Wasilewska, W. Dyga, Z. Drıg, An influence of occupational exposure to pesticides on the level of DNA damage induced in human lymphocytes (Polish group) by UVC and X-rays

M. Konopacka, O. Palyvoda, J. Rzeszowska-Wolny, Inhibitory effect of vitamin C on radiation-induced DNA damage in human lymphocytes in vitro

A. Cebulska-Wasilewska, W. Dyga, W. NiedŸwiedŸ, A. Panek, S. Krasnowolski, R. Marcos, C. Siffel, S. Piperakis, Comparison between influence of occupational exposure to pesticides in various countries on susceptibility and repair capacity of UV induced damage

J. Kusova, L. Dobias, M. Adamcik, J. Havrankova, I. Mikulenkova, T. Adamus, J. Burdova, J. Kubackova, H. Tomaskova, Metallurgical technologies and genotoxic risk

H. Lehocká, J. Kubina, Relationship between occupational exposure and genotoxic risk in department of clinical pathology

M. Lenarczyk, S. Murty Goddu, Dandamudi V. Rao, R.W. Howell, Induction of micronuclei in peripheral blood reticulocytes as a bone marrow dosimeter for determining exposure to incorporated radionuclides

£. Matulewicz, Maria Wide³, Dose rate effect on tumour cells measured by comet assay

J. Mierauskiene, Investigation of chromosome aberrations in occupationally exposed persons

M.G. Medeiros, A.S. Rodrigues, M.C. Batoréu, A. Laires, A. Zhitkovich, Rueff, Biomarkers of chromium exposure and effect: a comparative study in tannery workers and welders

A.K. Nersesyan, N.S. Vartazaryan, R.M. Arutyunyan, Micronuclei and other nuclear anomalies in exfoliated cells of gynecological cancer patients

W. NiedŸwiedŸ, S. Piperakis, A. Cebulska-Wasilewska, Influence of occupational exposure to pesticides on susceptibility to the induction of DNA damage studied with Comet assay and challenging dose (a Greek group)

A. Panek, W. Dyga, R.Marcos, A. Cebulska-Wasilewska, Monitoring of genotoxic effects in lymphocytes of people exposed to pesticides (a Spanish group)

E. Reszka, W. Wısowicz, K. Rydzyñski, Glutathione S-transferase gene polymorphisms in lung cancer patients

P. Rossner, Jr., B. Binkova, I. Chvatalova, R.J. Šrám, The effect of acrylonitrile exposure on the expression of p53 and p21WAF1 proteins in the blood plasma of occupationally exposed workers and in human diploid lung fibroblasts

S. Rudaitiene, K. Husgafvel-Pursiainen, Aberrant methylation in primary lung cancer

N. Ryabokon, R. Goncharova, Natural populations of murine rodents as model objects in studying transgenerational effects of chronic irradiation

G. Slapsyte, V. Samerdokiene, Cytogenetic monitoring of hospital workers occupationally exposed to low levels of ionizing radiation

J.P. Teixeira, S. Silva, J. Torres, Ma Conceiçao Azevedo, P. Neves, C. Gonçalves, O. Mayan, Biological monitoring of occupational exposure to styrene

S. Uleckiene, A.-V. Domkiene, The investigation of anticarcinogenic and antigenotoxic effects of original peptide Duodenine

A. Weso³owska, J. Ostrowski, Screening the human expression library for protein interaction with vacuolating cytotoxin (VacA) of Helicobacter pylori

A.Cebulska-Wasilewska, A. Wiecheĉ, I. Paw³yk, B. Binkova, R.J. Šrám, P.B. Farmer, Influence of Occupational Exposure to PAHs on the Induction and Repair of DNA Damage Induced by Challenging Dose and Evaluated by the Alkaline Version of the SCGE Assay

A. Wierzewska, E.Kasper, Z. Żabiñski, P.Moszczyñski, A. Cebulska-Wasilewska, Cytogenetic damage detected in lymphocytes of occupationally exposed to mercury vapours donors

L. Klimèáková , J. Zidzik, V. Habalová, J. Schmuczerova, J. Šalagoviè, I. Kalina, NAT2 gene polymorphism associated with susceptibility to bladder cancer in Slovak population independently on smoking